Saturday, August 31, 2019

Happiness for Our Life

HAPPINESS for OUR LIFE WHAT IS HAPPINESS: Happiness is thought of as the good life, freedom from suffering, flourishing, well-being, joy, prosperity, and pleasure. Are you truly happy? Do you even know what it means to be happy and what it takes to achieve happiness? These are important questions for anyone who is seeking happiness to ask themselves. I live my life to maintain my own happiness while trying my best to not cause unhappiness to anyone else. If you want to be happy you need to understand that you can be happy and that you should be happy.Many people make the mistake of believing that they don’t deserve happiness and accept their unhappy state as their destiny. The truth of the matter is that happiness, like anything else in life, needs to be nurtured. Two Faced I Smile When I’m Happy, I Smile more When I’m Sad FEW TIPS TO FOLLOW TO CREATE HAPPINESS IN OUR LIFE: 1. Understand what it is that will make you happy. Everyone has unique requirements for at taining happiness and what makes one person happy may be very different from what makes someone else happy. Revel in your individuality and do not worry about whether or not your desires are comparable to those of your peers. . Make a plan for attaining goals that you believe will make you happy. Your mood will very likely increase as your pursue your goal because you will feel better about yourself for going after something you value. 3. Surround yourself with happy people. It is easy to begin to think negatively when you are surrounded by people who think that way. Conversely, if you are around people who are happy their emotional state will be infectious. 4. When something goes wrong try to figure out a solution instead of wallowing in self pity.Truly happy people don’t allow setbacks to affect their mood because they know that with a little thought they can turn the circumstances back to their favor. 5. Spend a few minutes each day thinking about the things that make you happy. These few minutes will give you the opportunity to focus on the positive things in your life and will lead you to continued happiness. 6. It’s also important to take some time each day to do something nice for yourself. Whether you treat yourself to lunch, take a long, relaxing bath or simply spend a few extra minutes on your appearance you will be subconsciously putting yourself in a better mood. . Finding the humor in situations can also lead to happiness. While there are times that require you to be serious, when it is appropriate, find a way to make light of a situation that would otherwise make you unhappy. 8. Maintaining your health is another way to achieve happiness. Being overweight or not eating nutritious foods can have a negative effect on your mood. Additionally, exercise has been known to release endorphins that give you a feeling of happiness. 9. Finally, it is important to understand that you deserve happiness.Those who believe that they are not worthy of happiness may subconsciously sabotage their efforts to achieve happiness. If necessary, tell yourself each day that you deserve to be happy and remind yourself what steps you will take to achieve the happiness you desire. Happiness is hard to define but most people are aware of whether they are happy or not. Many people believe that happiness is a form of luck and that some people are destined to be happy while others are destined to be unhappy. The tips in this article are small but meaningful steps that you can take each day to lead you to true happiness. Happiness for Our Life HAPPINESS for OUR LIFE WHAT IS HAPPINESS: Happiness is thought of as the good life, freedom from suffering, flourishing, well-being, joy, prosperity, and pleasure. Are you truly happy? Do you even know what it means to be happy and what it takes to achieve happiness? These are important questions for anyone who is seeking happiness to ask themselves. I live my life to maintain my own happiness while trying my best to not cause unhappiness to anyone else. If you want to be happy you need to understand that you can be happy and that you should be happy.Many people make the mistake of believing that they don’t deserve happiness and accept their unhappy state as their destiny. The truth of the matter is that happiness, like anything else in life, needs to be nurtured. Two Faced I Smile When I’m Happy, I Smile more When I’m Sad FEW TIPS TO FOLLOW TO CREATE HAPPINESS IN OUR LIFE: 1. Understand what it is that will make you happy. Everyone has unique requirements for at taining happiness and what makes one person happy may be very different from what makes someone else happy. Revel in your individuality and do not worry about whether or not your desires are comparable to those of your peers. . Make a plan for attaining goals that you believe will make you happy. Your mood will very likely increase as your pursue your goal because you will feel better about yourself for going after something you value. 3. Surround yourself with happy people. It is easy to begin to think negatively when you are surrounded by people who think that way. Conversely, if you are around people who are happy their emotional state will be infectious. 4. When something goes wrong try to figure out a solution instead of wallowing in self pity.Truly happy people don’t allow setbacks to affect their mood because they know that with a little thought they can turn the circumstances back to their favor. 5. Spend a few minutes each day thinking about the things that make you happy. These few minutes will give you the opportunity to focus on the positive things in your life and will lead you to continued happiness. 6. It’s also important to take some time each day to do something nice for yourself. Whether you treat yourself to lunch, take a long, relaxing bath or simply spend a few extra minutes on your appearance you will be subconsciously putting yourself in a better mood. . Finding the humor in situations can also lead to happiness. While there are times that require you to be serious, when it is appropriate, find a way to make light of a situation that would otherwise make you unhappy. 8. Maintaining your health is another way to achieve happiness. Being overweight or not eating nutritious foods can have a negative effect on your mood. Additionally, exercise has been known to release endorphins that give you a feeling of happiness. 9. Finally, it is important to understand that you deserve happiness.Those who believe that they are not worthy of happiness may subconsciously sabotage their efforts to achieve happiness. If necessary, tell yourself each day that you deserve to be happy and remind yourself what steps you will take to achieve the happiness you desire. Happiness is hard to define but most people are aware of whether they are happy or not. Many people believe that happiness is a form of luck and that some people are destined to be happy while others are destined to be unhappy. The tips in this article are small but meaningful steps that you can take each day to lead you to true happiness.

Friday, August 30, 2019

Henry V: Ideal Christian King and Royal Bearing Essay

In King Henry V, King Henry is a man of Christian values and a solid foundation of moral principles. Through careful analysis of the entire play, one is able to confirm that he conducts himself with royal bearing. King Henry is â€Å"no tyrant but a Christian king† who is â€Å"a true lover of the Holy Church†. This highlights the idea that King Henry is of royal bearing as he acts in accordance with good Christian values. He questions whether he â€Å"may [†¦ ] with right and conscience make [a] claim to the throne of France†. Surely a man who only takes what he believes belongs to him is of an elevated moral standing and Christian divinity. King Henry says to his men at Harfleur that â€Å"[I]n peace there’s nothing so becomes a man as modest stillness and humility†. A ruler who practices and preaches such Christian values conducts himself with royal bearing. King Henry insists that â€Å"[he] would have all such offenders cut off† when news of Bardolph’s inexcusable crime reaches him. Executing a soldier and countrymen is no pleasant task. However, King Henry once again clearly demonstrates royal bearing and acts according to strong Christian values when making a decision regarding Bardolph. When [King Henry] speaks, [†¦ ] the mute wonder lurketh in men’s ears to steal his sweet and honeyed sentences†. He inspires his men at Harfleur to â€Å"imitate the action of the tiger [and] conjure up the blood†. Only a man of royal bearing preaching Christian values can arouse such patriotism in the hearts and minds of his followers. King Henry makes several references to God to ensure that he â€Å"delivers [his] puissance unto God†. He strongly believes in the idea that â€Å"God [is] before [†¦ ] and beside him in all his action†. Everything â€Å"lies [†¦ within the will of God†. These open references to God establish King Henry’s character as one of Christian spirituality and royal bearing. King Henry believes that â€Å"if you will France win, then first with Scotland begin†. He refuses to go to war with France unless England and its people are protected in his absence. Once again, King Henry proves he is of royal bearing. Therefore, through carefull analysis of the entire play, one is able to confirm that King Henry is a Christian king who conducts himself with royal bearing.

Cyberbullying Essay: Define, Prevention and Effects

The model answer for  Cyberbullying Essay. Structure of Essay:Introduction –  Definition of Cyberbullying, instances, how cyberbullying begins, age group cyberbullying is most active.Effects of Cyberbullying –  Mental & Physical, Isolation, effect on friends and familyHow To Prevent Cyberbullying  Ã¢â‚¬â€œ Steps to be taken by parents, school authorities, governmentConclusionCyberbullying Essayco.uk/wp-content/uploads/2016/01/maxresdefault-2-1024x576.jpg" alt="Cyberbullying Essay" width="755" height="425" />What is Cyberbullying?1. IntroductionDefinition: Cyberbullying  is the act of bullying in the form of digital media or devices. It is a modern term for bullying, which emerged with the rise of the internet and mobile devices. Cyberbullying takes place on digital devices like cell phones, computers, and tablets. It can take the form of texts through SMSes, online chat forums, social networking sites and gaming forums. Acts such as posting, sending or sharing negative content that is harmful, mean or obscene, defines cyberbullying. Cyberbullying can also take the form of some illegal physical activities and unlawful behavior. The internet is the place where cyberbullying is most common. Social media sites like Facebook, Snapchat, and Instagram, to name a few are the most common sites for cyberbullying. With the introduction of free messaging apps, the instances of cyberbullying have increased. The rampant use of mobile phones has brought cyberbullying at the tip of fingers and cyberbullies make use of these technologies to prey on potential victims.Examples and Instances of Cyberbullying:Born in 1996, Amanda Todd was like any other teenager with dreams and a fascination for the internet. She met a stranger online who flattered her and convinced her to go topless. The stranger took pictures of her and kept stalking here for several other shows. When she tried to fight back the photos were sent to her family and friends. After which, she was abused and disrespected in school by her friends. Going into major depression and anxiety, she tried taking her own life several times and finally succeeded. A month before she killed herself she released a video telling her story which became viral after her death and people realized how dreadful it was. This is a  prime example of how cyberbullying can destroy innocent.How Cyberbullying Begins:We live in a world dominated by technology and not only teenagers, but adults too are addicted to the use of electronic devices and the internet. The internet has played a major role in connecting the entire world and individuals from across the continents. Cyberbullying starts in the virtual world. The cyberbullies enjoy the anonymity and the infinite reach of the internet to prey on unsuspecting and potential victims. Once they get hold of something personal of the victim, they start to either blackmail or harass them.Age Group in Which Cyberbullying is Most Active:Victims of cyberbullying are most commonly from the age group of 12 to 18. Teenagers get attracted to the online glamour and the attention that they get from strangers. The virtual fandom is what they seek, and they tend to do things for gaining followers on social sites such as Facebook and Instagram. But older victims can get caught in it too. School students are easy to target, and if they have a bully within they school then, it becomes more difficult to escape the bullying.2. Effects of CyberbullyingMental And Physical Effects:Cyberbullying can result in increased distress for the victims along with increased anger and frustration. They will vent this out on various oc casions, and it might force them to get addicted to the virtual world where they can have several identities to hide their real one. The victims will automatically have lower self-esteem since that is the sole purpose of bullying. Prolonged bullying will lead to severe withdrawal from their family and friends. They will begin favoring isolation most of the time and indulging in harmful activities like drugs and alcohol.Feel Alone and Isolated:Most of the victims are forced to stay away from any and all social sites in a bid to escape the cyberbullies. They separate themselves to feel a sense of protection form the outer world. Most of the victims do not reveal anything to their parents or family members to disappoint them. To escape the bullies, they may try to change their schools or home, but they are hardly able to escape the clutches of the tormentors. Most of the time the victims would give up fighting the bullies and kill themselves to escape the pain and horrors of the continuous cyberbullying.Disinterested in Attending School:If a schoolmate bullies a  Ã‚  kid, then the first way for him to avoid the bullying is to stop going to school. He/she would choose to skip classes to escape the tormentors temporarily. The feeling of embarrassment and humiliation from the fellow students will prevent the kid to return to school and have a healthy school life. Their grades will start dropping, and most of the time they will drop out.Victims Might Get ill:Apart from the usual mental and physical effects of cyberbullying, it can take a toll on the health of the victim. Symptoms like headaches and stomach problems are very often seen in the victims. It can also result in issues that are caused due to stress like various skin conditions and stomach ulcers. Due to binge eating or skipping meals several weight issues can arise, and they might have to deal with insomnia and other sleeping disorders.3. How To Prevent Cyberbullying?Identify Victims:Identifying victims may be a challenge if they are introverts initially, but every victim leaves off some prominent clues to their condition and their troubles. Isolating oneself and staying alone is one such clue. If they seem to be depressed all the time or are usually anxious and angry, then it may signal that they are going through some form of bullying.Steps to be Taken by Parents and Family Members:The primary step is to prevent the kids from coming in contact with these harmful and dangerous predators that are always prowling about for a victim. Try to keep the computer in a common area and stay updated with all the latest websites and trends on the internet. Teach them about the internet and how to use it responsibly and safely. Communication is an essential step in getting to know about their situation. Talking to the child will help in building trust, and they will be more comfortable in sharing their troubles with the parents. Once they share what is happening, try not to overreact and blame them for the incident. Ask them to be strong and let them know that they will always have their parents by their side. Cyberbullying is an actual threat and not a childish thing. Realizing the gravity of the situation is important in solving it.Steps to be Taken Schools and Colleges:The faculties should be aware of any student showing the signs of being a bully or being bullied. If they have any concern, then they should delicately handle the situation and inform the authorities to take over the case. Schools should have counselors to assist the students and help them overcome any tough situation.Steps to be Taken as a Country and Laws Necessary:Laws against cyberbullying have been in place for quite some time though they were introduced after many such cases were highlighted. The rules are designed to protect the victim and keep the case information confidential. The law enforcement authorities should be proactive in the investigation of such a situation unlike in the Amanda Todd case where the police wer e slow in taking action and did not consider the matter important which ultimately cost the life of an innocent kid.  4. ConclusionCyberbullying is a serious issue, and it can be truly horrific to go through such a difficult period. Staying strong and fighting back is the only option against these bullies. It’s possible to get over it and live your life again. Seeing a counselor can help the victims handle the incident in a better and a safe way. Life does not get over if you are a victim of cyberbullying and it is possible to fight back.Statistics Regarding Cases of Cyberbullying:According to the US Department of Justice, in the year 2010 to 2011, 9% of the total students aged between 12 and 18 were cyberbullied. A survey conducted the year 2012; it was found that almost 55.2% of students belonging to the LGBTQ community were cyberbullied. Over the past years the rate of cyberbullying has increased, and in the year 2016, a survey found that the number of people who have faced cyberbullying at least once in their lifetime doubled from about 18% to nearly 34%. This is evidence of how rampant cyberbullying is becoming. By the year the percentage of high school students who were bullied had become 5%, and the percentage of middle school students who had become victims of cyberbullying was 24%. Through the help of several surveys, it was found that girls were targeted more than boys in cases of cyberbullying.

Thursday, August 29, 2019

To what extent the laws on foreign invested enterprises in China have Essay

To what extent the laws on foreign invested enterprises in China have transformed in line with China' WTO membership - Essay Example Moreover, the ‘dual tax system’ has led towards imposition of different tax rates that are applicable to FIEs and Chinese enterprises. FIEs in China possess favourable tax treatment in comparison to domestic Chinese enterprises. The FIEs are also facilitated with special tax rates as well as deduction of taxes along with tax holidays 1(LI 2008). The People’s Republic of China (PRC) Enterprise Income Tax Law, from the beginning of the year 2008 has developed a unified tax system that is applicable for both domestic Chinese enterprise and FIEs. In accordance with the revised PRC law, it has been viewed that an income tax charge of 25 percent is applicable for all business enterprises of China2. In December 2001, China acceded towards significant World Trade Organisation (WTO) that deals with trade regulations between different nations, ultimately changed the scenario of the business law environment of China3. In this regard, WTO had devised significant reforms for d ifferent FIEs in China that emphasised upon minimising tariff rates as well as opening new sectors for foreign investment. Moreover, WTO has also reformed the legal system which includes protection of intellectual property as well as control of foreign exchange4. The discussion intends to emphasize upon examining the historic background, development and features of Chinese law governing the forms of organisation belonging to FIEs. Moreover, the WTO impact upon the transformation of Chinese business law will also be portrayed in the discussion. Main Body Historic Background and Development of FIEs The historic background and the development of Chinese law governing the forms of organisations of FIEs can be segregated into three phases that have been discussed hereunder. The Initial Stage (1979-1986) In the year 1979, a law on Chinese-Foreign Joint Ventures was approved and it was the first law of the State Council of China that is related to foreign investment consumption. The Chines e State Council from the year 1979 to 1980 had provided favourable as well as flexible policies with regard to foreign investments. In order to develop the Chinese economy as compared to Soviet Union, China developed a legal system based on Soviet Union legal framework. In this similar context, it was viewed that Hong Kong as well as Macao was regarded to be the major foreign investors in China and investments were mainly made in manufacturing units as well as hotels which are mainly labour intensive5. Steady Development Stage (1987-1991) In the year 1986, the State Council instigated a law relating to the development of foreign investments for the motive of minimising issues between domestic Chinese enterprises and FIEs. Moreover, the law helped in eliminating the problem of currency exchange for foreign investors and it also facilitated the foreign investors and export businesses to reap significant benefits. During this stage, foreign investments increased in export enterprises a s well as manufacturing industries along with high technology industries and its expansion in many business areas was also been observed 6. Accelerated Development Stage (1992-1999) In the year 1993, it has been observed that the amount of foreign inve

Wednesday, August 28, 2019

Social Network and Religion Essay Example | Topics and Well Written Essays - 500 words

Social Network and Religion - Essay Example Nowadays, Social Networking Service (SNS) has become the most powerful communication technology in addition to being the fastest medium to use especially when people want to share their life stories. Specifically, Facebook, Twitter, and Snapchat are the most powerful social networking services that influence a larger number of users in the United States. Before the Internet was invented, television and radio were the major communication technologies in terms of mass media that were intended to reach a large number of audiences. People who could not attend the church on Sunday used to take advantage by watching TV and listening radio. However, the overall use of the word â€Å"media,† has declined because of a new idea of mass media. This new media has been defined as being associated with the Internet and interplays amongst technology, sound, and images like YouTube that shows visual test with sound in the online world. While at the same time, the definition of new media chang es consistently and therefore, is a mixture concept of both new and mass media, which is SNS, and has an enormous impact on our religious society and culture. On the other hand, these new communication technology services may sound useful but nevertheless have a dangerous effect on the religious aspect like bittersweet. Everyone can be an evangelist through an activity of SNS. For instance, people can share their personal story or even anything that is associated with the religion anytime immediately. In case of Facebook, users can interact with others simply by pressing â€Å"like† or sharing someone’s post, which is one click away communication. Through SNS activity; people who are in religion have a regressive aspect that they are more likely to overlook the real religious activity. In Latin phrase, â€Å"extra Ecclesiamnullasalus† means there is no salvation outside

Tuesday, August 27, 2019

The Blues and Jazz Research Paper Example | Topics and Well Written Essays - 1750 words

The Blues and Jazz - Research Paper Example The epilogue and the first chapter of the book ‘invisible man’ serve as the basis for this research. Foundation of Jazz Music Jazz music emerged from African Americans in New Orleans As most music scholars would put it, jazz music was among the first American genres that influenced global music. In essence, many share the ideas that this form of music developed after the civil war especially in America (Clark 56). On the other hand, jazz musicians also used this music genre as a platform for advocating for civil rights and equality for the African American minority group. It was a subtle way of dealing with social injustices for which others considered as stereotyping since it mainly targeted those of white color. In essence, music serves as an avenue for communicating across one’s ignorance’s and hatreds hence connecting people for the betterment of the society. With this attribute in mind, many jazz musicians played their music for a purpose that was grea ter than entertainment but as a way of bringing unity for the oppressed. Ellison’s use of Jazz and Blues in the book ‘Invisible Man’ serves as a way of self-expression. ... This explains why the narrator chooses to listen to such music in his underground hole as he strived to peer deeper into the invisible man that he received little attention from the whites. Although many jazz artists were from New Orleans, this does not limit the themes to this location only. On the contrary, many African Americans formed the audience of those jazz artists because the themes expressed reflected the feelings and experience of all of them. Famous jazz artists included Louis Armstrong, Duke Ellington, John Carter, Wyntons Marsalis, and Wadada Leo Smith. All these artists expressed the feelings of African Americans. They made use of a variety of symphonies that defined their style of the mainstream African music (Clark 80). Jazz emerged at a time when the civil rights movement in America was operating under a compelling impetus. The popularity of jazz music was to contribute greatly to anti-racial themes. This is evident because the song ‘What did I do to be soâ₠¬â„¢ that the narrator in ‘Invisible man’ listened to in the underground hole is a reflection of the sentiments harbored by many of the Black people against racism. Louis Armstrong had remixed the song to address the racism theme that was affecting the Africans Americans. It is worth noting that the emergence of jazz was a motivation of black people to extend their outcry against the institutionalization that slavery had caused. Jazz music formed one of the avenues through which African American could convey their quest for equal treatment. In addition, this was the period shortly after the world war and Africans demanded inclusion in all systems as

Monday, August 26, 2019

Discussion Board Essay Example | Topics and Well Written Essays - 500 words - 4

Discussion Board - Essay Example A disaster recovery plan includes such modules and applications which saves data in case of failures. A disaster recovery plan assesses all aspects of data and puts proper procedures in place to make the data as secure as possible. I have sent the proposal regarding creating, testing, and maintaining a proper disaster recovery plan to ensure data safety. Although it will take some time and money to create and implement an effective disaster recovery plan, but the benefits associated with the implementation of plan will overshadow the costs. Nothing is more important to an IT based company than data security because every function of an IT company needs analysis of data. Therefore, implementation of disaster recovery plan will be very beneficial for the company as it will not only make the data secure but also will prevent disruption to the computing environment. I have also proposed the use of intrusion detection software to the company in order to prevent unauthorized access to the sensitive data. Installation of such system will not be cost; rather it will provide a strong mechanism to deal with the issues of data stealing and unauthorized access. Some of the main benefits of installing such system include defense against data stealing, prevention of network hacking, continuous network monitoring, update of firewall settings, and many other benefits. Therefore, both of my proposals should be accepted because they will ensure data security to the company. A hot site is a part of a disaster recovery service using which a company can continue its activities in case of a disaster. Benefits of hot sites include availability and convenience for the customers whereas disadvantages include cost and complexity (Moore, 2011). A cold site is also a part of a disaster recovery service but using these, customers need to install necessary equipments to perform data related activities in case of a

Sunday, August 25, 2019

Assignment essay and discussion Forum in Sociology Week 10

Assignment and discussion Forum in Sociology Week 10 - Essay Example re many African Americans, Blacks and Hispanics in America who are being discriminated against either based on ‘real or alleged physical characteristics such as skin color, hair texture, eye shape, or other subjectively selected attributes† or â€Å"on the basis of cultural or nationality characteristics† (kendall, p. 255-56). Such groups of people are very often ill-treated and are negated of equal opportunities and are looked down as inferiors. Instances on stratification based on race and ethnicity is visible in almost all aspects of life; the â€Å"color line† that negated African American baseball players of entry into the national team for centuries is the best example for stratification based on race and ethnicity. These sorts of stratification have given rise to the emergence of dominant and subordinate groups in the American society. Prejudice, segregation, discrimination, stereotyping, and ethnocentrisms are the salient features of a society where racial or ethnic bias prevails. When it comes to racism and ethnicity, questions such as whether people sort others based on physical appearance or individual traits come to one’s mind. It is also interesting to note whether everybody classifies people in the same way. The PBS link on sorting people shows that people are classified even based on physical traits such as skin color, finger prints and blood type. Based on these three types of physical traits, people are categorized into American Indians, Asians, Blacks, Hispanic/ Latino, and Whites. The conclusion drawn is that there are many ways to sort people and that â€Å"race is a social idea† the categorization of which is not always â€Å"based on nature, but on our ideas† (Sorting People: Conclusion). One should also notice that there is considerable degree of disparity in income distribution, percentage of poverty and educational attainment among these various races. The US Census Bureau of 2003 shows that the social standings of Africa n Americans and

Saturday, August 24, 2019

Linguistics and Media Term Paper Example | Topics and Well Written Essays - 1250 words

Linguistics and Media - Term Paper Example It is the short form or ellipsis of a word or phrase. It is also called initialisms: using the initials of the words and compressing it into short form, such as in the word LOL taking L from ‘laughing’, O from ‘out’, and L from ‘loud’. 2. Abbreviations: 1. f2t? : free to talk 2. cul8r: see you later 3. Ruok? = Are you ok? 4. Xlnt! = excellent 5. 2day: today 6. B4: before 7. thN: then/than The word formation process used here is pseudo-acronym. These appear as acronym or abbreviation but they actually are not and they do not stand for anything. They are used because, when pronounced as intended, they resemble the sounds of other words. They are words or initials used to give the sound of an entire word or phrase. Such as the words given above: Before = b4. B. Frames: In the excerpt, the main structuring frame is the semantic frame, it is a coherent structure of concepts; a word, idea or a concept cannot give meaning unless all elements of the str ucture are present (Gelbukh 307-312). The extract is a pep talk, an encouraging speech that motivates the people and congratulates them on choosing the right option for themselves and their country. The major points of the passage were conveyed in empathic tones, choosing to use ‘we’ rather than ‘I’, it emphasizes the importance of the people and that only by standing together they can overcome obstacles. It constructs a mental state of unity and importance in listeners Attributes and values of the frame: 1. Attributes: The attributes in this frame is the victory that is being celebrated which brings with it a lot of challenges also. 2. Values: The individual instantiation is the phrase â€Å"I have never been more hopeful than I am tonight† - it represents the individual symbolization of the attribute: the victory that comes with challenges, that the individual is hopeful that they can be solved C. Metaphors: A conceptual metaphor refers to understa nding one idea or concept with the help of another idea. An idea or thought is explained in terms of another, they are a way of communication and shape our way of thinking and acting. Conceptual metaphors have become a regular part of our communication. The American public is hungry for a national policy that is coherent and cohesive: The conceptual metaphor in this sentence is â€Å"hungry for† which shows want and need of the American Public and the extent and depth of their need. This expresses the ferocity of their need so that the reader has an acute idea of the profundity of their wish. Last week I was depressed, my spirits sank and I was really low. But my best friend has cheered me up since, and I am feeling up now: The cognitive or conceptual metaphor in this sentence is â€Å"spirits sank and I was really low. I am feeling up now† it is trying to express emotions in terms of directionality. The term low refers to being down, less, dejected, morose, depressed etc.; sank also assumes the same meanings. It shows the direction of his emotions that they are low or have sunk, meaning he is very depressed. Opposing low is up; which has a positive meaning or perception in the human mind, up depicts a state of growth, happiness, acceleration, betterment etc. so saying that I am up now means that I am feeling better or happier now. We launched this campaign not far from here on a beautiful June day. It has been an extraordinary journey. The metaphor is â€Å"it has been an extraordinary journey†; journey is a source domain in this sentence, the word from which we draw the

Friday, August 23, 2019

Rationale for choosing the two companies and industries based on your Essay

Rationale for choosing the two companies and industries based on your industry and fundamental analysis - Essay Example During last financial year i.e. in 2010, companies have experienced higher growth and hence, there had been higher dividend yield (Vellacott and Cruise, 2011). UK economy has been experiencing slower economic growth but it steady and expected to grow much higher by 2012. However, due to sluggish economic recovery, Confederation of British Industry (CBI) has claimed that â€Å"expected national output to increase by 1.3% in 2011 compared to the 1.7% it had been predicting three months ago† (Elliott, 2011). During 2007-2009, overall GDP growth was negative due to global crisis, but during 2010, these parameters have taken an increasing trend that will make stock market grow in coming years. Industry Analysis Telecommunication UK telecommunication industry is highly developed in terms of industry polices and market exposures like demand. For example, during end 2010, nearly 4.8 million and 33.1 million were the users of mobile broadband and 3G mobile (Ofcom, 2011). Figure 6 portr ay that mobile ownership has been grown from 50% to 91% since last 10 years and figure 7 shows that there also have been growth in broadband connection. The market report also signifies that there has been a slight decline in annual revenue of telecommunication industry as presented in the following figure. However, it is expected that, during 2011, industry revenue is expected to grow by 2% to ?35.6 billion and annual growth rate will grow rate nearly 3%-4% p.a. By the end of 2015, market is projected at ?40.7 billion i.e. â€Å"equivalent to cumulative growth of 17% in real terms compared with 2010† (MBD, 2011). Pharmaceutical Multiple new product development led by technological advancement and government’s initiatives are expected to bring rapid growth in healthcare industry. Comparing to other manufacturing industries, pharmaceutical has shown a better performance. The above figures show UK’s Pharmaceutical trade, there has been significant growth in this i ndustry since last decades. The overall import and export has increased and medical pharmacy products are the highest gainer. Company Analysis Vodafone Plc Vodafone Group is one of leading global telecommunication company, and in UK Vodafone’s performance has been remained better than its rivals. A key comparison of Vodafone with its key rivals British Telecom and Telefonica SA are given below. The overall performance of Vodafone has been better than its rivals in terms of profit margin and its sales and stock returns its performance has remained quite better. Moreover, with lowest debt-equity ratio, it also has lower risk level. The following table depicts the financial highlights. Vodafone’s net profit margin and sales volume are very high that clearly signifies its better financial performance. Its shareholders have also gained a higher return on their investments as earning per share and dividend payout ratio are attractive for making investments. The key ratios of Vodafone indicate that financial condition is very stable than its key rivals. Return on equity is also

Thursday, August 22, 2019

The Wisdom of Whores Essay Example | Topics and Well Written Essays - 2000 words

The Wisdom of Whores - Essay Example Despite WHO identifying the challenges related with the spread of HIV and establishing a global program on AIDS, it made an error of associating HIV with consequences on both social and economic development of human beings. According to the author, the issue development led to drawing interest in the matter from the World Bank while UNICEF, UNESCO and others felt that the concern equally touched on their dockets. From that wide approach and push to address the same issue, the challenge at hand was never solved as there occurred change of direction where rather than solving the challenge at hand, counter accusations and defense was traded. Through presenting the counter accusations that delayed the process of addressing AIDS challenge, the Chapter captures the general idea of the book that argues against the myths attributed to HIV/ AIDS. Stine (2014) presents a twenty two year chronological of AIDS pandemic where he attributes the responsible parties in addressing the challenge to fa cilitate rather as opposed to fighting the challenge. The author believes that failure of fight against HIV/AIDS resulted from failure to address the issue at the right time and assigning the role to a particular body. These assertions from the Pisani are true considering that delay in fighting the challenge even led to introduction of other mechanisms of beating up the issue. However, with limitations to access of some necessities, achievement of the goal became unrealizable. In the second chapter, Pisani discusses her mission in Indonesia where she was engaged in a new role carrying out a study that will help the government understand how and the reasons behind the spread of HIV in Indonesia. Through developing an effective national surveillance system, the government intended to learn the trend of HIV spread on a yearly basis. Like in her arguments about failing to do the right thing at the right time, â€Å"there lacks a point of getting information of HIV

Online Education vs Traditional Education Essay Example for Free

Online Education vs Traditional Education Essay Nowadays, with the technology furtherance and the increased use of the internet, online education has gained significant acceptance and popularity all over the world. Not too long ago, online education offered no competition to traditional education . Today, with more choices being offered online, traditional education is now facing a number of challenges in every level. This has as a result, the competition between those two educational programs, having to compare the advantages and disadvantages of both methods of study, being measured since those methods are means for us to expand our opportunities and advance our careers. In the following paragraphs, I will expand the differences and similarities between online education and traditional education and compare their characteristics. Online education is defined as the creation and proliferation of the personal computer, the globalization of ideas and other human acts, and the use of technology in exchanging ideas and providing access to more people. Audio, video, computer, and networking technologies are often combined to create a multifaceted instructional delivery system. The fundamental method to unite the distance learning instructor with the distance learner is the network. Networks suitable for distance learning implementations include satellite, cable modem, digital subscriber lines (DSL), and wireless cable, (Collins, 2002). According to an article, students need to monitor their progress to the technology evolution and adjust their strategies for learning based on their development. Plus they need to control a time management schedule in order to complete online learning activities in the proper timeframes, (Orellana, 2009, p. 470). Online education requires time management skills and requires self-discipline. It takes patience, time and energy to be able to do that with success. The online student must be able to balance school, work, and a family. However, proper management must be maintained in order to balance all of the responsibilities and be successful in all areas. Online education gives you the opportunity to pursue quality and affordable education without interrupting your personal or professional life. It allows more flexibility and you are able to manage your study schedule according to your comfort and availability of time. So, you can program your personal life respectively with your studies thanks to the online education’s special mode of learning. This gives to the students the comfort to study from their homes, workplaces or anywhere they are in the world. What’s more is that, many distance programs allow to their student to complete the coursework any time during the day, while still adhering to overall deadlines. Online education has lower cost. It is considered more affordable than traditional because there are no transportations costs, no need to buy textbooks and you do not need to rent a place to live close to the university. Also, online education programs offer scholarships, financial aid packages, including loans and grants, exactly as the traditional education institutions. Another characteristic that cannot be ignored is communication. In online education programs you can communicate with your tutors anytime you want, anywhere, with any way you prefer to communicate (with the technology you have available). Online educational techniques are not only flexible and interactive, but at the same time interesting as. Besides, the fact that the student has straight access to an assigned professor or lecturer, allows each student to learn with the methods that work best for them. Another feature of online education is that if you are not a social person, an online school may be the right choice for you. You do not have to deal with meeting up new people. Moreover, a big part of people choose to follow the online education route for the quantity of material they are able to find, or because the tutors are more obtainable via internet than they would be in a normal classroom setting. At the same time, there are some similarities which can be seen in an online and traditional system of education. First of all, assignments are a part of learning, whether you choose to attend classes online or in a traditional system, you will be assigned homework to complete. The assignments are expected to be done about the course you are enrolled in and delivered them to your personal tutor between the deadlines. A second similarity between online and traditional system is that the reference material text books are common for both systems. Even though a course may be taken online, text books are still central to learning relevant information and many of the students are using the electronic books. In traditional education, students have to buy the books because they study from them in the class and home. Thirdly, tests and exams are used to test your understanding and retention of a particular topic/subject. It is usually given to students during the academic semesters including midterms. Even though you may study in an online course, you have to expect to be required for course exams. Exams may be given online, or you may be required to visit the institutions campus to complete the exam. In both education systems, students have common information to study thus they have common exams. Last but not least, academic instructors make themselves available to students to help them learn the required material. Whether you are attending school online or on campus, professors are likely to have office hours, where you can get clarification on an issue or otherwise communicate with your professor. In both the types of classes, teachers will be available to help the students in any matter. Alternatives ways to contact them would be email. Traditional education, also known as back-to-basics, conventional education or customary education, refers to long-established customs found in schools that society has traditionally deemed appropriate. Some forms of education reform promote the adoption of progressive education practices, a more holistic approach which focuses on individual students needs and self-expression. In the eyes of reformers, traditional teacher-centered methods focused on rote learning and memorization must be abandoned in favor of student-centered and task-based approaches to learning. However, many parents and conservative citizens are concerned with the maintenance of objective educational standards based on testing, which favors a more traditional approach. (Wikipedia,2011) First of all, school is a main place which you can meet up people from different places in your community. Friendship is too important, no-one can live without having a friend around. School is the most important place for humanity to learn. It is very important for everyone because is a place that people can build up strong relationships with each other and also is a place to learn a lot of information. In addition, communication is easy because everyone is on the same building the same hours. So, it is easier for students to study together and help each other. You can ask questions whenever you deal with difficulties either to your teacher either to your classmates because you are all in each other lives. Lastly, study at school is easy for the teacher to take care of the students, help them and get to know how he/she should behave according to the student. Whenever a student has questions or difficulties, he or she can ask teacher right away, also students don’t need to wait for a long time just for a question. Now, comparing the traditional education with online education system there are and some differences too. Starting with online education, firstly, students do not have the opportunity to socialize with friends and do not communicate with other people. Also, students are spending too many hours in front of one screen and that can causes back pain and eye problems. Moreover, if you have limited access to internet (connections are slow) and you do not know how to use technology then you probably fail to your lessons. Continuing with the traditional education, enables students to have more creativity and be more sociable thus they can cooperate and collaborate with each other and the teachers. It is easier to communicate because you have face-to-face contact. Furthermore, teachers can provide instant feedbacks to the students and motivate them to succeed and teach them about discipline. In my opinion, REFERENCES: * Collins, (2002), Definition of online education * Schutte, J. G. , (1996),â€Å"Virtual Teaching in Higher Education: The new intellectual superhighway or just another traffic jam? †, California State University, Northridge. * http://www. acadenia. com/education-articles/Online-education-traditional. asp * http://www. dagorret. net/difference-between-online-education-vs-traditional-education/ * http://lyzreblin. blogspot. com/2012/02/online-education-vs-traditional. html The challenges of online education * Jeni Johnson, (2012), Online Education Vs. Traditional Education, April 4 * Wikipedia, (2011), Traditional education, May, * (Orellana, Hudgins, Simonson, 2009, p. 470). * Elise Wile, Advantages and Disadvantages of Traditional Education, eHow Contributor * Similarities Between Online Traditional Classes,eHow. com In conclusion, although online education have some beneficial impacts for students, but traditional education provide better place for students to study and socialize with friends. As well as some people limitations to access technology can occurs students can not have education. An online education program can be a very rewarding experience for those individuals wanting to earn a degree in a non-traditional education environment. Studies have shown that students who are mature, self-motivated and organized are extremely successful in their online learning pursuits. By allowing students to control where and when they study, online programs are best-suited to those individuals who possess strong time management skills and are independent learners. Online learning also offers prospective students who are employed or have children and other family commitments the opportunity to earn a degree without sacrificing other important areas of their lives. Younger students, such as elementary or secondary school age, and students who are dependent learners, may find it difficult to assume the responsibilities required by an online program. Ultimately, you make the final decision as to which education program is right for you.

Wednesday, August 21, 2019

Advantages And Disadvantages Of Joining A Currency Union Economics Essay

Advantages And Disadvantages Of Joining A Currency Union Economics Essay Currency Union are a group of countries that share a single currency. There is a misconception that currency unions are a product of the 20th century globalization, but it is not true. They have existed since the times of Roman, but still they havent been adopted as a global financial system. The reason being despite it having many advantages it has few disadvantages as well. I will discuss these advantages and the disadvantages in the first part of my essay. While in the second part I will show some light on the current heated debate about UK joining the Euro Zone. Transaction cost:- The most essential advantage connected with changing to a single currency was the removal of the need to change currency . Savings are very large because of the elimination of the transaction cost connected to the exchanging currency, the taxes for countries that have most of the exports to the European counties only. The significant decrease in the cost of exports will be most useful for small scale business to achieve economies of scale. By switching to the euro, members of the EMU were expected to save as much as $30 billion a year (The Euro, the European, pp. 154), :- Daniel Portone. Investment:- As there is low transaction cost there is large amount of investment because companies now this is one of the most important decreases in the cross border investment. This has lead to large cross border investment like in France the foreign direct investment has increased from 12% to 18%. The disappearance of the cost of transaction and the introduction of the common currency makes the money market deeper and integrated. The major financial institutes are being listed in the Euro, which in turn attracts potential investors to gain confidence in different EU financial markets . The market combination provides various links to dilute the risk in the EMU. If we assume that the French and German bond and equity markets are fully integrated, it will facilitate the adjustment to asymmetric shocks (see Figure 1). When France is hit by a negative shock, companies there make losses and that drives down stock prices of these companies.- Jean Monnet.which bring the profit to germen investors ,thus the boom in germany brings profit to French . A very similar mechanism also works through the fully integrated bond market, Jean Monnet Exchange rate stability (Common Currency):- Common currency generates a platform to judge the price relationship, make price difference more noticeable and helps to equalise it across borders.- Jean Monnet .Along with the removal of the need to change currency, there is also problem with the volatility of the exchange rates also. When the rate fluctuates it also affects the profitability of the company and increasing the risk which in turn decreases the net investment into the country. Thus to stabilise the situation it is useful for a company to enter in a currency union. .. having the same currency can boost trade by a factor of three. Canada again provides the example : inspire of close proximity to the US and similarities in culture , Canadian provinces trade twelve to twenty times more amongst themselves than with the US states The common currency provides the member nation to compare the prices efficiently . The poor regions would never become richer simply by devaluing its cur rency repeatedly. On the contrary the associated high inflation would introduce economic distortion and reduce its average real income, :- Professor Alec Chrystal Free movement of workers :- Free mobility area of the labour helps the countries to prevent from an asymmetric shock which is the result of inflation in one country A and a recession in country B . If there is mobility of labour ie they can move freely this will lead to release the inflation in A and increasing employment of the people in B. For example, workers from Indonesia, Malaysia, Philippines, and Thailand account for 10 percent of the employment in Singapore. Emigration has been as much as 2 percent of the labor force of the sending countries- (http://www.adb.org/Documents/ERD/Working_Papers/WP012.pdf ) The prevention of competitive devaluations and speculation:- The Monetary unions protect the member countries damaging effect of competitive devaluation of the currency which may lead to steeling the business of the other . But is any country which try to do this with the monetary unions has an adverse effect of high inflation. Other advantages of joining the currency union are as follows. The country gets an access to larger markets and thus increasing the overall income of the country. It also reduces the effect of shocks from exterior instability to an individual country. Joining the currency union is very important for those countries which lack internal control . This allows free movement of goods and sevices without any obstacles. This also keeps peace between the nation as they now that they are all interdependent on each other . the one of the most important advantage is it it will increase the tourism in the countries as there is easy movement and no currency changes . Disadvantages of currency union Loss of sovereignty: This means that country adopting the Common currency has to give up the Monetary policies to the body who is controlling the union . like in the case of European union all the 12 countries had to give up their monetary rights to the European central bank with decides the monetary policies for all the nation . Its most biggest disadvantages come during the crisis when the situation are different in all the different countries and cannot be handled in the same way. Like in a case of sudden increase in the unemployment the governments income will decrease as taxes are not paid so the government will have to increase the taxes which will lead to further disaster decrease in the interest rates during the crisis will help some but will adversely affect the other . So it is very difficult to be in an currency union . In the United States, Texas could not avoid a recession in the wake of the 1986 oil price fall, whereas demand for Sterling changed in the light of the ne w oil price, adjusting the exchange rate downwards.- http://news.bbc.co.uk/1/hi/special_report/single_currency/25081.stm Cost of adopting the new currency: The adopting of new currency will have a very huge cost to the economy. These are like Such changes include educating customers, changing labels, and training staff, changing computer software and adjusting tills.- http://news.bbc.co.uk/1/hi/special_report/single_currency/25081.stm Lower inflation and reduced transactions costs of trade provide gains, while the inability to respond to idiosyncratic asymmetric shocks generate losses.:- Andrew K. Rose1 New negative cross-border spillovers of fiscal policy:- a national fiscal expansion raises the demand for savings, ceteris paribus pushing up the long-run interest rate and discouraging investment. In an integrated capital market strengthened by monetary unification, this effect will spread to other countries, imposing a negative externality. A monetary union may also generate new negative spillovers. An increase in domestic government purchases, in affecting the demand for domestic products, raises local inflation, thereby pushing up average euro-area inflation and forcing the ECB to contract monetary policy for the entire area. Further, a national fiscal expansion may cause an appreciation of the euro, thereby undermining the external competitive position of all union members.- (http://www.voxeu.org/index.php?q=node/4305) The other disadvantages of the Monetary unions are as follows, the one of the biggest disadvantage is the difference in languages with in turn leads to the decrease in the mobility of labour Language in Europe is a huge barrier to labour force mobility. This may lead to pockets of deeply depressed areas in which people cannot find work (http://news.bbc.co.uk/1/hi/special_report/single_currency/25081.stm.). The countries in the currency union also lose the ability to cope with the external shocks. It have to leave it on itself so be rectified with in todays time is very difficult. Should Britain join euro Britain is one of the biggest financial hub in the world, which is also the worlds largest hub for currency trading . Britain does the maximum currency trading . Britain from the beginning has been independent and has been flourishing . But it was a real shock to the Britain when EMU was formed and the biggest threat . After reading the list of journals , it is very difficult to say whether or not Britain should join the Euro or not . There are many arguments and thoughts over it and would like to bring them forward to you . I would first like to bring forward all the positive aspects of the Britain joining the euro with the real facts about it . There has been a significant decrease in foreign direct investment in Britain after the formation of the EMU . Britains share of the foreign direct investment coming into Europe has fallen by a half (see Table 3). In 2001 the Netherlands received more of this investment than Britain.:- Richard Layard, Willem Buiter, Christopher Huhne, Will Hutton, London as mentioned is a important financial hub, but the pound has little to do with it. Where most of the trading is done mostly in dollars in most of the transaction , but the euro outranked the pound ,where euro was involved in 41% and pound in just 24 % of the transactions (as seen in the table 1). The other thing is that this financial centre employ about 1,50,000 people creating  £10- £15billion annual invisible exports . If the UK exercises its opt-out, long-term damage would be inflicted on the City, which will ultimately lose its pre-eminence to Frankfurt or even Paris, in part because trading in the Euro will be focused within its area of operation:- Brian Burkitt The continues increase in the instability will decrease the attractiveness of Britain has as a destination of capital flow. The stock of euro-denominated corporate bonds nearly tripled between 1998 and 2001, to 1.2 trillion Euros. This clearly shows the euro-zone has reconstructed its business which has increased the annual cross border foreign direct investment by 4 fold. Britain almost has its 50% of its trade with the EMU, which is shown in table 2, so it would be better for the UK to join the euro and thus reduce its cost of import and exports .During November 2002 the Chief Executive of Ford UK specifically stated that euro/sterling exchange rates were damaging the profits of the company:- http://www.fpma.scot.nhs.uk/euro_pros_cons.pdf The British consumers will be now able to compare prices all over Europe . This will end the phenomenon of rip-off Britain that allowed coca-cola to charge double here what it charges in Spain, or Ford to charge 43% more for a focus than in Denmark. :- Christopher Huhne . From long time the MNCs new that Britain is the Treasure Island and the consumer are willing to pay high price. Britain chance to exploit the Asia and the America is by joining the bigger currency thats the Euro

Tuesday, August 20, 2019

Relationship between SMC1A and Chromosome Related Disease

Relationship between SMC1A and Chromosome Related Disease 1. Introduction[A1] The Structural Maintenance of Chromosomes 1 alpha (SMC1A) gene is located in Xp11.22-p11.21, consisting of 25 exons and 24 intron. SMC1A gene encoding a core subunit of the cohesin complex, which is essential to sister chromatid cohesion. SMC1, SMC3, SCC1 (also known as MDC1 and RAD21) and SCC3 (also known as SA2 and STAG2) subunits could interact with each other and form a ring-shaped cohesin complex [1-3]. As is known, central component of the cohesin and condensin complexes are required for conversion of interphase chromatin into mitotic-like condense chromosomes[4]. Structural Maintenance of Chromosomes (SMC) proteins are core component of the cohesin and condensin complex and essential for chromosome condensation during DNA replication and chromatid segregation of the genome in all organisms. They are also involved in checkpoint responses and epigenetic silencing of gene expression[5]. Cornelia de Lange syndrome is a dominantly developmental disorder with multisystem abnormalities including slow growth before and after birth, characteristic facial features, upper extremity defects, hirsutism, gastroesophageal dysfunction and cognitive retardaion. The incidence is as high as one in 10,000 to 30,000 newborns. Both sexes have the same phenotypic variability. To date, the three genes, NIPBL, SMC1A, and SMC3 involving in chromosome function, gene regulation and double-stranded DNA repair, could cause CdLS when mutated[6, 7]. Six in ten of the probands with CdLS have heterozygous mutations in NIPBL gene, whereas 5% have mutations in SMC1A and SMC3 genes [6, 8]. Eleven different SMC1A mutations in 14 unrelated patients have been reported. All patients had a mild to moderate CdLS phenotype [8-10]. In several decades, we focus on the relationship between SMC1A and chromosome related genetic disease. In recent years, we found that SMC1A may play a key role in tumorigenesis. Sun. M et al. determined that the effects of SMC1A knockdown on the cell cycle and apoptosis of lung adenocarcinoma cells. The results indicated that SMC1A is a novel oncogene, which modulates lung cancer cells in their proliferation and migration capabilities through arresting cell cycle at G0/G1 phase and promoting apoptosis [11]. The similar conclusion also was found in glioblastoma cells [12, 13]. However, SMC1A functions as a novel oncogene in human prostate cancer metastasis and progression has still not been reported. 2. Materials and Methods 2.1. Patient Samples All of the patient samples were obtained from the Urinary Surgery Department of Shanghai Changzheng Hospital, Shanghai, China. This study was approved by the Clinical Research Ethics Committee of Shanghai Changzheng Hospital, and the informed consents were acquired from all of the subjects. 2.2. Reagents and antibodies DMEM (cat no.12430-054), F12 (cat no. 21127022) and RPMI-1640(cat no. 11875-093) medium and fetal bovine serum (cat no. 10099-141) were purchased from GIO (Grand Island, NY). TRIzol Reagent was from Invitrogen (Carlsbad, CA, USA). Giemsa was from Chemicon International (Temecula, CA). M-MLV Reverse (cat no. M5301)Transcription was purchased from Promega (Madison, WI, USA). Oligo-dT(18) was synthesized by Sangon Biotech (Shanghai, China). Terraâ„ ¢ qPCR Direct SYBR ® Premix (cat no. 638318) was from Takara (Otsu, Japan). Anti-SMC1A antibody (cat no. SAB4300451) was from Sigma-Aldrich (Munich, Germany). Mouse anti-GAPDH (cat no. sc-32233), Goat anti-Mouse IgG (cat no. sc-32233) and goat anti-rabbit IgG (cat no. sc-2030) were from Santa Cruz Biotechnology (Texas, USA). All the other chemicals were of analytical grade. 2.3. Cell culture Human embryonic kidney cells 293T, Human prostate cancer cell lines PC-3, DU145, LNCap, and 22RV1 were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). 293T were cultrued in DMEM containing 10%FBS. PC-3 and DU145 cells were maintained in F-12 medium supplemented with 10% FBS, 100U/ml penicillin and 100ÃŽ ¼g/ml streptomycin. 22RV1 and LNCap cells were incubated with RPMI-1640 supplemented with 10% FBS, 100U/ml penicillin and 100ÃŽ ¼g/ml streptomycin. LNCap cells were maintained in Corning Corning ® CellBIND ® Surface cell culture flasks (Corning, cat no. #3289) for a better attachment efficiency. All cells were cultured in a humidified incubator at 37oC under 5% CO2 atmosphere and used for analysis during exponential phase of growth. 2.4. RNA interference The synthesized 21-bp oligonucleotides encoding SMC1A-specific shRNA held the sequence 5’-TAGGAGGTTCTTCTGAGTACA-3’. The sequence of the negative control shRNA oligonucleotides was 5’-TTCTCCGAACGTGTCACGT-3’. The oligos were annealed and ligated into pH-L vector (Hollylab, Shanghai, China) through NheI/PacI to generate pH-Lv-shSMC1A and pH-Lv-shCon. The resulting constructs were confirmed by sequencing. 2.5. Recombinant lentivirus Transduction PC-3 or DU145 cells were plated at 5Ãâ€"104 cell/well in 6-well plates. After 24 h of culture, lentivirus recombinant encoding shRNA against SMC1A was added at a multiplicity of infection (MOI) of 50 into F-12 basic medium. After 6h incubation, the cells were added with complete growth medium replacing the basic medium containing the lentivirus. Then, 5 days post-transfection, gene reporter (EGFP) expression was examined using fluorescent microscopy (Olympus, cat no. CKX41). 2.6. Quantitative real-time RT-PCR analysis of SMC1A mRNA expression Total RNA was extracted using TRIzol reagent according to the manufacturer’s instruction. 2ÃŽ ¼g total RNA was used to synthesize the first strand of cDNA using M-MLV Reverse Transcriptase. Real-time PCR reactions using Terraâ„ ¢ qPCR Direct SYBR ® Premix were run on Takara TP800-Thermal Cycler DiceTM Real-Time System. The following primers were used: SMC1A: 5’- AGCGAAAGGCAGAGATAATGG-3’ and 5’-GGTAGTCAAGAGGCAAGAAGG-3’; ÃŽ ²-actin: 5’- GTGGACATCCGCAAAGAC-3’ and 5’-AAAGGGTGTAACGCAACTA-3’. Thermal cycling condition were 1 min at 95 °C followed by 45 cycles of 95  °C for 5 s, 60 °C for 20 s, read absorbance value at the extension stage. The data was analyzed with Takara Thermal Dice Real Time System software Ver3.0. SMC1A relative mRNA levels was calculated using the 2-ΔΔCt method with normalization to ÃŽ ²-actin. And the conditions 2.7. Western blot analysis of SMC1A protein expression Cells were washed twice with ice-cold PBS and suspended in cell lysis buffer (2% Mercaptoethanol, 20% Glycerol, 4% SDS in 100mM Tris-HCl buffer, pH 6.8), and incubated for 15 min on ice. After centrifugation at 12,000 g for 15 min at 4oC, the supernatants were collected, and the protein content were measured using BCA protein assay kit. Equal amounts of protein were subjected to SDS-PAGE. After electrophoresis, blots were transferred onto PVDF membrane using an electro-blotting apparatus (Tanon, Shanghai, China). The membrane was blocked with TBST buffer containing 5% nonfat milk at room temperature for 1 h, and incubated with the primary antibodies in the blocking solution at 4oC overnight. After 3 washes with TBST buffer, the membrane was incubated with horseradish peroxidase (HRP) -conjugated secondary antibody (1:5000) at room temperature for 1 h. The signals of detected proteins were visualized by Pierce ECL western blotting detection kit (thermo scientific, USA). GAPDH protein level was used as an internal control to verify equal protein loading. 2.8. MTT assay Cell proliferation was evaluated by MTT assay. Exponential growth phase cells were plated at a final concentration of 2000 cells/well in 96-well plates and cultured for five consecutive days. MTT (10 µl, 10mg/ml) was then added, followed by incubation for another 4 hr at 37oC under humidified 5% CO2 atmosphere. The MTT was removed and addition of 150ÃŽ ¼l DMSO. Optical density (OD) of each well was measured at 490 nm using an ELx808 Absorbance Reader (Bio-Tek Instruments, USA). 2.9. Colony formation assay Cell growth and survival ability was also determined by the plate-colony-formation assay. In brief, 200 transfected cells were plated in 6-well plates. Cells were cultured for 14 days at 37oC under humidified 5% CO2 atmosphere. Culture medium was changed at 3-day intervals. Afterward, cells were incubate in 4% paraformaldehyde for 30 min at room temperature. The colonies were stained with Giemsa for 15 min, then washed with ddH2O and air-dried. The number of colonies (>50 cells/colony) was counted. 2.10. Flowcytometry Analysis Cell cycle distribution was assessed by propidium iodide (PI) staining. Briefly, the transfectedcells were harvested by trypsinization, centrifuged at 250 g for 5 min, washed twice with ice-cold PBS, and fixed in 70% ethanol at 4oC or -20oC for at least 1 h. Cells were collected and resuspended in PBS containing 100ÃŽ ¼g/ml RNase A and 40ÃŽ ¼g/ml PI, and then incubated at 4oC for 30 min, in dark. Cells were analyzed by flow cytometry using a FACSCalibur flow cytometer (Becton-Dickinson, San Jose, CA). The percentage of the cells in sub-G1, G0/G1, S, and G2/M phases were analyzed using ModFit (Verity Software House, Maine, USA) software. 2.11. Migration assay To explore the effect of SMC1A in the migration of prostate cancer cells, 24-well transwell chamber with 8.0ÃŽ ¼m pore polycarbonate filter inserts (Corning, cat no. #3422) was performed. In the upper chamber of each transwell, cells were suspended in serum-free F-12 containing 0.2% BSA. And F-12 supplemented with 10% FBS was added in each lower chamber. Then, the inserts were incubated at a 37 °C, 5% CO2/95% air incubator for overnight and the cells that had not penetrated the filters were removed. The migrated cells attached to the bottom side were fixed in 4% paraformaldehyde for 10 min and stained in0.1% crystal violet for 30 min, rinsed in PBS and examined under a bright-field microscope. 2.12. Tumorigenesis assay The influence of SMC1A silence on the tumor development of prostate tumor in vivo was examined. DU145, DU145-Lv-shCon or DU145 Lv-shSMC1A at 5 Ãâ€" 106 per mouse were injected subcutaneously into 4 weeks old Balb/c nude mice (n = 10 per group, Shanghai Laboratory Animal Center, Chinese Academy of Sciences, China). The development and growth of solid tumors were monitored by measuring tumor size using a vernier caliper in a blinded fashion every five days for a 27-days period. The tumor volume was calculated using a standard formula: tumor volume (mm3) = width (mm)2Ãâ€"length (mm)Ãâ€"0.5. At the end of the experiment, all mice were sacrificed and individual tumor weights were measured using a electronic balance. All the animal experiments were approved by the Animal Care Committee of the Second Military Medical University. 2.13. Statistical analysis The statistical analyses were performed with Graphpad Prism 5.0 software. The values are expressed as the mean of at least three different experiments  ± S.D. The results were analyzed by Student’s t-test, and P 3. Results 3.1. Expression of SMC1A in prostate cancer tissue and prostate cancer cells. To study the function of SMC1A in prostate cancer, we first analyzed its expression pattern in prostate cancer tissues. As shown in Fig 1A, SMC1A was strongly stained in prostate cancer tissue with a clear subcellular localization in the cytoplasm and nucleus of abnormal prostate epithelial cells. Then, the expression level of SMC1A was further analyzed by western blot, which showed that SMC1A was upregulated in prostate cancer tissues (Ca) compared to the adjacent normal tissues (N) (Fig 1B), implying a possible correlation between SMC1A and prostate cancer. To find a cell model for further investigation of SMC1A’s function in prostate cancer, we first analyzed the expression of SMC1A in four commonly used prostate cancer cell lines. Both WB and qPCR indicated that SMC1A expressions were elevated in PC-3, DU145 and 22RV1 cells in comparison to the androgen-sensitive LNCap cells (Fig 1C and D), which had less aggressiveness than the other three cell lines. We also found that S MC1A expression level was negatively correlated to AR expression level (Fig 1C and E), suggesting that SMC1A might be correlated with the malignancy of prostate cancer and involved in AR signaling. 3.2. Lentivirus-mediated knockdown of SMC1A in prostate cancer cells. As PC-3 and DU145 cells expressed much higher levels of SMC1A, they were used for further investigation. Both PC-3 and DU145 cells were untreated or transducated with Lv-shCon or Lv-shSMC1A. The transduction efficiencies were above 90% in both cells confirmed by fluorescent microscope (Fig 2A). WB analysis demonstrated that Lv-shSMC1A efficiently knocked down SMC1A expressions in PC-3 and Du145 cells (Fig B and C). Q-PCR results indicated that SMC1A was down-regulated more than 80% and 90% in PC-3 and DU145cells respectively. 3.3 Down-regulation of SMC1A inhibited cell proliferation in prostate cancer cells. After confirming the knocked down efficiency of SMC1A, PC-3 and DU145 cells were analyzed for cell growth rate with MTT assay. As shown in Fig 3A and E, cells transducted with Lv-shSMC1A displayed suppressed growth rate in comparison to the control or Lv-shCon transducted cells. The cells were then seeded onto 6-well plates for the analysis of colony formation ability. PC-3 and DU145 cells transducted with Lv-shSMC1A formed colonies with much smaller sizes compared to the control and Lv-shCon transducted cells (Fig 3B and F).The colonies formed in 6-well plates were photographed (Fig 3C and G), and counted (Fig 3D and H). The results suggested that both PC-3 and Du145 cells showed impaired colony formation abilities after SMC1A knockdown,indicating a pivotal role of SMC1A in regulation of prostate cancer cells proliferation.

Monday, August 19, 2019

Animal Abuse and Cruelty to Animals Essay -- essays research papers

In years of humanity's existence, the role of the animals can be considered as one of the factors that have greatly shaped human culture. The contribution of the animals to society is of much importance as it has generously benefited the people since the distant past. Animals have always been a source of food, clothing, and subservient workforce for humankind. These animals have also taken part in the religious customs, either for religious sacrifices or worshiping, done way back the earliest times. Moreover, People have long been reliant on their unique contributions to balance out nature and the much needed companionship that they provide. Truly, animals have taken an influential part in the continuous struggle of mankind for survival. Through the years, animals have still been used for the same purposes and means that benefited mankind. But in attune with the continuously developing world, innovative ideas and radical thinking accordingly emerged. With these breakthroughs, the thought of recognizing animals as sentient beings and not mere possessions only have started to unfold. Although it was supposed that such thinking has began as a system of belief back in the Indus Valley Civilization, it was not until the early 1800?s when the first deliberate approach in promoting the animal interests was done (Cruelty to Animals, 2001). Since then, the deed of imposing cruelty to animals has been considered as one of the major moral disputes ever dealt with in the society. The moralistic justification of such acts is often immoral, unjust and offensive. It generally refers to a treatment that causes unacceptable suffering or harm to animals (Cruelty to animals, 2001). Although having similar moral grounds for opposin... ... is really happening to the animals. These encouragements have greatly helped in demolishing animal abuse in different parts of the world. But contrastingly, such developments appear to be deficient in the Philippines. Although various organizations have also been established here in the country that struggle for same purposes, still abuse of animals is still widely practiced by Filipinos. The government has also hardly given appropriate attention to such immoral problems in the society. Conceivably, Filipinos lack the awareness and willingness to engage themselves to such campaigns. I just do hope that the continuous cruelty to animals, not only here but as well as in every part of the world, be eventually put to end. After all, mankind?s continued existence is partly owed to these animals and it is but proper only that we give them befitting respect and valuing. Animal Abuse and Cruelty to Animals Essay -- essays research papers In years of humanity's existence, the role of the animals can be considered as one of the factors that have greatly shaped human culture. The contribution of the animals to society is of much importance as it has generously benefited the people since the distant past. Animals have always been a source of food, clothing, and subservient workforce for humankind. These animals have also taken part in the religious customs, either for religious sacrifices or worshiping, done way back the earliest times. Moreover, People have long been reliant on their unique contributions to balance out nature and the much needed companionship that they provide. Truly, animals have taken an influential part in the continuous struggle of mankind for survival. Through the years, animals have still been used for the same purposes and means that benefited mankind. But in attune with the continuously developing world, innovative ideas and radical thinking accordingly emerged. With these breakthroughs, the thought of recognizing animals as sentient beings and not mere possessions only have started to unfold. Although it was supposed that such thinking has began as a system of belief back in the Indus Valley Civilization, it was not until the early 1800?s when the first deliberate approach in promoting the animal interests was done (Cruelty to Animals, 2001). Since then, the deed of imposing cruelty to animals has been considered as one of the major moral disputes ever dealt with in the society. The moralistic justification of such acts is often immoral, unjust and offensive. It generally refers to a treatment that causes unacceptable suffering or harm to animals (Cruelty to animals, 2001). Although having similar moral grounds for opposin... ... is really happening to the animals. These encouragements have greatly helped in demolishing animal abuse in different parts of the world. But contrastingly, such developments appear to be deficient in the Philippines. Although various organizations have also been established here in the country that struggle for same purposes, still abuse of animals is still widely practiced by Filipinos. The government has also hardly given appropriate attention to such immoral problems in the society. Conceivably, Filipinos lack the awareness and willingness to engage themselves to such campaigns. I just do hope that the continuous cruelty to animals, not only here but as well as in every part of the world, be eventually put to end. After all, mankind?s continued existence is partly owed to these animals and it is but proper only that we give them befitting respect and valuing.

Sunday, August 18, 2019

Australian Life :: essays research papers

Australian Life Life in the Bush The Australian life style was portrayed as one of the bushman although it was not . The life of a typical Australian was really the city life . The bushman was one with no classes and he treated every person as equal . A middle class person was the same as a working class man . The bushman was a very trust worthy and helpful character he would help a stranger as if it was his friend and if that friend needed anything he would be glade to ofer him every thing he has . The bushman lead gypsy like lifestyle with the constant moving and roaming round the country side . They would often walk or ride horse back from place to place with their tent and billy and camp out over night when they had no where to stay . The bushman where very much like aborigines they tracked and did not need a compass or a map they knew plants and trees . They called this bushcraft . A bushman would cook , clean , wash his cloths and patch his pants this made them very independent . If a bushman was ordered or commanded he would say " Are you talking to me or the dog ? " they treated everyone equal and wanted to be treated equal . They dressed in tweeds , flannel tops , with blue jumpers in the cold and yellow oilskin jackets in the rain . They wore hard wearing blucher boots and broad felt hats . The bushman would often have a drinkdown the pub . They often told stories to each other as their past time . The bushmans homes were made from slabs of bark , green hide . The houses were simple and didn't always show advantage . Nor dose it have to , the bushman's home (bellow) is there to shelter him . In the 1880's writers and painters portrayed the bush life as better then the city life . This bought on the image that all Australians lived in the bush . City Life The city life of Australia was not recognized as much as the bush life although most of the population of Australia were present in the cities . The cities in the mid 1880's were walking cities only the few wealthy were able to afford the private horse-drawn transport . The wealthy with their transport were therefore able to live out side of the city center . The working class worked and played short distances away from home . Australian Life :: essays research papers Australian Life Life in the Bush The Australian life style was portrayed as one of the bushman although it was not . The life of a typical Australian was really the city life . The bushman was one with no classes and he treated every person as equal . A middle class person was the same as a working class man . The bushman was a very trust worthy and helpful character he would help a stranger as if it was his friend and if that friend needed anything he would be glade to ofer him every thing he has . The bushman lead gypsy like lifestyle with the constant moving and roaming round the country side . They would often walk or ride horse back from place to place with their tent and billy and camp out over night when they had no where to stay . The bushman where very much like aborigines they tracked and did not need a compass or a map they knew plants and trees . They called this bushcraft . A bushman would cook , clean , wash his cloths and patch his pants this made them very independent . If a bushman was ordered or commanded he would say " Are you talking to me or the dog ? " they treated everyone equal and wanted to be treated equal . They dressed in tweeds , flannel tops , with blue jumpers in the cold and yellow oilskin jackets in the rain . They wore hard wearing blucher boots and broad felt hats . The bushman would often have a drinkdown the pub . They often told stories to each other as their past time . The bushmans homes were made from slabs of bark , green hide . The houses were simple and didn't always show advantage . Nor dose it have to , the bushman's home (bellow) is there to shelter him . In the 1880's writers and painters portrayed the bush life as better then the city life . This bought on the image that all Australians lived in the bush . City Life The city life of Australia was not recognized as much as the bush life although most of the population of Australia were present in the cities . The cities in the mid 1880's were walking cities only the few wealthy were able to afford the private horse-drawn transport . The wealthy with their transport were therefore able to live out side of the city center . The working class worked and played short distances away from home .

Saturday, August 17, 2019

Project Management Chapter Notes

These tools provide basic project management features and generally cost I than $200 per user. Smartened and tablet APS are available for much less, but the y often have Limited functionality. Low end tools are often recommended for small projects and single users. Most of these tools allow users to create Gaunt charts, which cannot be done easily us inning current productivity software. Midrange tools: A step up from low end tools, midrange tools are designed to handle larger rejects, multiple users, and multiple projects.All of these tools can produce Gaunt c harts and network diagrams, and can assist in critical path analysis, resource allocation, project tracking, and status reporting. Prices range from about $200 to $1,000 per user, or less per MO nth for online tools. Several tools require additional server software for using workup feat rues. Microsoft Project is still the most widely used project management software in this ca destroy, and t has an enterprise version, as desc ribed earlier and in Appendix A.Students and deed actors can purchase software like Microsoft Project at reduced prices from sites like www. Journey yet. Com ($139. 95 for Project Professional 2010 in 2012), and anyone can download a trial verse on from Microsoft's Web site. Many other suppliers also provide trial versions of their product Weighed tools: Another category of project management software is high end tools, sometimes referred to as enterprise project management software.These tools provide robust c abilities to handle very large projects and dispersed workups, and they have enterprise an d portfolio management functions that summarize and combine individual project information t o provide an enterprise view of all projects.

Beowulf and Illiad Essay

Stories like Beowulf and the Iliad seem hard to believe, far-fetched, and exaggerated even though they can relate to a cultures past. Even so, both stories have great examples of fighting, victory, and glory. â€Å"Now though I may win my perfect glory†¦Ã¢â‚¬  (Line 64 Homer) This quote helps show the desire Achilles has for glory. â€Å"I’ve never known fear, as a youth I fought in endless battles. I am old, now, but I will fight again, seek fame still, if the dragon hiding in his tower dares to face me. (Line 607-611 Beowulf Poet) This quote shows Beowulf has a similar desire for fame and glory, along with the confidence to be victorious. Both relate the tales of a hero, and as we follow them, we learn about them in the process. The story of Beowulf seems too good to be true in a sense. As you read, you notice that every woman is beautiful, every warrior is good and every cause is worth risking life and limb for. What is realistic is that every human being wants to be loved and accepted while also being the hero. King Hrothgar is a fatherly example that deserves the love that Beowulf has for him. Hail, Hrothgar!†¦ My youth have been filled with glory. Now Grendel’s name has echoed in our land: Sailors have brought us stories of Herot, the best†¦When the moon hangs in skies the sun had lit, light and life fleeing together. † (Line 140-148 Beowulf Poet) This quote shows the fondness that the king and Beowulf have for each other, and it shows a comparison of light and life. In the case of Achilles in the Iliad, everything is a bit more believable. The theme of life and death is brought to the reader’s attention when Patroclus is killed. As a result, Hector is also killed because of Patroclus’s death. Achilles isn’t perfect, along with other characters such as Hector and King Agmemmon. These faults make this story more realistic and believable, while also adding truth to the story. Bad decisions are made, such as Achilles allowing his good friend, Patroclus, to enter battle and be killed. â€Å"My greatest friend is gone: Patroclus, comrade in my arms, whom I held dear above all others- dear as myself – now gone, lost; Hector cut him down, despoiled him of my own arms, massive and fine, a wonder in all man’s eyes. (Line 13-18 Homer) In this quote is an example of life and death and how it made Achilles wants revenge on Hector. This also gives Achilles a type of â€Å"power† to choose whether to take Hector’s life. Beowulf is humble, and although he has the personality of a hero more than Achilles, he does not see his own value or his own significance. Achilles, on the other hand, could not be more proud. His awareness and consideration of his own skills and his own value are an obvious difference to the humility and mildness of Beowulf. Do not attempt to keep me from the fight, though you love me; you cannot make me listen. † (Line 70 Beowulf Poet) This quote shows an example of Achilles attitude toward himself and others when it comes to a fight. This also shows how Beowulf has more good in him then Achilles. Beowulf having a personality with great goodness, makes the decision to fight the greatest evil (Grendel) with only his hands. â€Å"My hands alone shall fight for me, struggle for life against the monster God must decide who will be given to deaths cold grip. (Line 172-175 Beowulf Poet) This quote supports Beowulf’s fight against evil, and how the fight will determine life and death. Beowulf and the Iliad are stories of great heroes. They take us through their stories and battles while teaching the reader about them along the way. The Iliad is easier to believe, while Beowulf seems too good to be true. Both stories relate to the cultures past and teach us about each character. Beowulf and the Iliad battle through evil and relate to each other through life and death.

Friday, August 16, 2019

Marriage/Chana Miller Essay

Fully explain and concisely illustrate two (2) of the â€Å"Theoretical Perspectives on Families† discussed in your text (pp. 37–49). Use families presented in television programs, the movies, novels, or the Bible to illustrate the selected perspectives. The functionalist perspective focuses on stability and cooperation and emphasizes the importance of the family in maintaining the stability of society. The functionalist theory states that marriage is a microcosmic replica of the larger society, and the family fills six basic needs for the survival of society. Family life is organized in ways that are useful or functional for society. Despite being antiquated, the breadwinner-homemaker family is an efficient way to organize family life. The husband works outside the home while the wife does the housework and child care. The functionalist perspective is a male-dominant perspective (Strong et. al. 2011). In modern times, feminists have attacked patriarchy view and many women resisted male domination. The Feminist Perspective argued that the functionalist view is inadequate and idealized. It stated that gender defines social roles and cultural characteristics. Yet these roles have no biological reason behind them but are culturally dictated; therefore, they are socially constructed. These roles appear to be constructed to give men power. Experience of living in a family is different for women than it is for men. Families can be sources of social inequality, especially reinforcing the inequality of women. Women having economic power can lead to many challenges within the family, and within society (Strong et. al. 2011). Independence is a good quality for feminists, but not for functionalists. Since families involve domination and struggle, there must be compromise and good communication or there can be many problems, leading to domestic violence and other such abuse. During the 1950s, the Cleavers on the television show â€Å"Leave It to Beaver† epitomized the American family. In 1960, the majority of American households were like the Cleavers: made up of a breadwinner father, a homemaker mother, and their kids. During most of the 1950s and 1960s, white middle-class families dominated programs. The 1960s, however, began to showcase more structural variability, with an increase in families headed by a single widowed parent, such as in The Andy Griffith Show. (Fleahman et. al. 2009) Today, â€Å"traditional† families with a working husband, an unemployed wife, and one or more children make up a very small percentage of the nation’s households. And as America’s families have changed, the image of the family portrayed on television has changed accordingly. Today’s television families run the gamut from two-career families to two single mothers and their children and an unmarried couple who cohabitate in the same house. Another factor reshaping family life has been a massive influx of mothers into the work force. As wives have assumed a larger role in their family’s financial support, they have felt justified in demanding that husbands perform more child care and housework. Feminism has also been a major force that has transformed American family life. The women’s liberation movement attacked the societal expectation that women defer to the needs of spouses and children as part of their roles as wives and mothers. The larger mainstream of the women’s movement articulated a powerful critique of the idea that child care and housework were the apex of a woman’s accomplishments or her sole means of fulfillment (Strong et. al. 2011). Historically, television has promoted a traditional family model with wise parents, little serious conflict, and mostly conforming behavior. Families on television during the 1950s and much of the 1960s talked with each other, and parents always helped their children through adolescence. Although the 1970s had a number of sentimental portrayals, such as â€Å"Little House on the Prairie† or the still popular â€Å"Brady Bunch,† it also experimented with more diverse relationship patterns in such favorites as â€Å"All in the Family† and â€Å"The Jeffersons. † In â€Å"All in the Family. † family members were likely to ignore, withdraw, and oppose one another, in addition to showing support and caring. (Fleahman et. al. 2009) During the 1980s, â€Å"The Cosby Show† dominated public perceptions of family portrayals with an enviable family. Primetime soap operas such as â€Å"Dallas† and â€Å"Dynasty† explored the seamier side of extended families. The end of the 1980s saw a more cynical view of the family in such comedy hits as â€Å"Roseanne† and â€Å"The Simpsons. † By the 1990s family relationships were again portrayed more positively in terms of psychological health on shows such as â€Å"Family Matters† and â€Å"Home Improvement. † (Fleahman et. al. 2009) Although conflicts in family programs have increased rapidly from the late 1970s, family members almost always successfully resolved the conflicts by way of positive, constructive, and pro-social communication.

Thursday, August 15, 2019

Nike Case

Nike Case 1. Should Nike be held responsible for working conditions in foreign factories that it does not own, but where subcontractors make products for Nike? No, I do not believe Nike should be responsible for working conditions in foreign factories. I do believe that there should be working standards in place and adhered to but I do not believe that is Nike’s responsibility. Nike is a business in order to run a successful business one must abide by good business practices including respecting others beliefs and values.It would be no different than if a Nike employee another employee how to raise their kids. As long as they are abiding by the laws governed in that their own country people are free to raise their children how they please. 2. What labor standards regarding safety, working conditions, overtime, and the like, should Nike hold foreign factories to: those prevailing in that country, or those prevailing in the United States? Nike should uphold the standards prevail ing in the particular country.If there are issued regarding safety, working conditions, overtime, etc, they should be discussed through separate entities, for instance the United Nations. 3. An income of $2. 28 a day, the base pay of Nike factory workers in Indonesia, is double the daily income of about half the working population. Half of all adults in Indonesia are farmers, who receive less than $1 a day. Given this, is it correct to criticize Nike for the low pay rates of its subcontractors in Indonesia? It is not appropriate to criticize Nike for low pay rates.According to salary. gov, the average American income is 46,326. If a company from United Arab of Emirates came exports products from a store in the U. S. and paid the works 105,623 (equal to the percentage raise Nike is paying in Indonesia), Americans would be grateful. 4. Could Nike have handled the negative publicity over sweatshops better? What might it have done differently, not just from a public relations perspectiv e, but also from a policy perspective? Yes, Nike could have handled the negative publicity better.For instance Andrew Young should have brought is own interpreter into the factories. There is no way of knowing what is actually being said and the motives behind a foreign interpreter. Nike took the right steps when it mandated hours worked per week, minimum wage per governing country, and raising the child labor laws. They set emplace standards and followed up with them. When they found businesses not adhering to the regulations they increased them, and gave the business a clear definition of what the regulation mandates.I don’t think Nike could/should have done anything else. 5. Do you think Nike needs to make any changes to its current policy? If so, what? Should Nike make changes even if they hinder the ability of the company to compete in the marketplace? No, I do not believe Nike needs to make changes to its current policy. The fact that they even implanted policy shows th eir character. If Nike did make changes and couldn’t compete in the market place thousands of Americans would be upset about losing their favorite sports brand, and millions of people would be out of jobs.The failure of Nike to compete in the market place could literally mean the down turn of foreign economies. 6. Is the WRC right to argue that the FLA is a tool of industry? The WRC is correct in stating FLA is a tool of the industry. However, the businesses involved are exactly that, businesses. The FLA was an appropriate step in the right direction for the pursuit of better business practices by other countries while maintaining the best business relationship possible. Disrespect, is not only a great way to lose business, but wars are literally started over it.FLA takes the times to look at the beliefs, and values of the companies it is working with and not just â€Å"do what they feel is the right thing to do†. 7. If sweatshops are a global problem, what might be a global solution to this problem? The United Nations should handle the sweatshop issue. That way it is not just the U. S. attacking, or implementing their business structures on other countries. The United Nations is specifically designed to handle global issues. Having multiple nations give their input and suggests would be an incredibly helpful and compromising tool for employees and employers in all countries. Nike Case Corporate Finance Nike, INC: Cost of capital 1. What is the WACC and why is it important to estimate a firm’s cost of capital? Do you agree with Joanna Cohen’s WACC calculation? Why or why not? Definition of WACC (Weighted Average Cost of Capital): WACC is basically the average of the cost of finance (debt and equity). Since a company’s assets can be financed by debt or equity, WACC can show the averages of the costs involved in the sources of financing. These costs are then weighted by the users of the information as required in a specific situation.This shows how much both debt holders expect to pay in interest and how much return the shareholder can expect to receive, for each dollar of financing (Investopedia, ND). The calculation of the cost of capital is one of the important elements that decide the enterprise value. The value of the enterprise can significantly change when the percentage of cost of capital changes in the business model, with the cost of ca pital representing the expected return for shareholders. We disagree with Joanna’s WACC calculation for following reasons: The calculations of WACC and DCF can be effected as they are subjective by her human judgment.Even though there are no right answers to make these decisions, our team disagrees with some of the assumptions Joanna Cohen made. i. ‘Ratio of debt financing’ and ‘Ratio of equity financing’ It has to be applied the market value because current shareholders’ expected return has to be reflected. Both ratios should be calculated not by using ‘Book Value’ but ‘Market Value’. ii. Cost of Debt Cost of Debt can be calculated with the current yield publicly traded in the market, because we are projecting the future cash flows. Joanna calculated this by using historical data.However cost of debt should be calculated using current YTM of debt. iii. Cost of Equity Joanna calculated cost of debt by using following CAPM formula: Cost of Equity = 5. 74% (20 year Treasury bond) +0. 80 (Average Historic Nike beta) *5. 9% (Average premium of the market over Treasury) =10. 5% When calculating the beta, using the most current beta is better than using the average, because the current beta reflects the most recent environment of Nike stock. 2. If you do not agree with Cohen’s analysis, calculate your own WACC for Nike and be prepared to justify your assumptions. i. Ratio of debt financing’ and ‘Ratio of equity financing’ Market value of debt = 5. 4 + 855. 3 + 435. 9 = $1,296. 6million Market Value of equity= There is no information about market value of debt. We will use ‘Book Value’ $1296. 6million Market Value of equity= Share price ($42. 09) * Shares outstanding (271. 5million) =$11,427. 4million Ratio of debt financing=1,296. 6 / (1,296. 6+11,427. 4) = 10. 19% Ratio of equity financing=11,427. 4/ (1,296. 6+11,427. 4) = 89. 81% WACC=9. 81%*89. 81%+7. 168 %* ( 1-38%)*10. 19% =9. 26% ii.Cost of Debt Market value of debt should be: Current price of debt: $95. 60 Coupon rate: 6. 75%(semiannual) =coupon $3. 375 per 6month Period to maturity: 20 years =40 period Face value: $100 YTM (=cost of debt) =3. 584% (semi annual) =7. 168% (annual) iii. Cost of Equity Using CAPM formula Cost of Equity = 5. 74% (20 year Treasury bond) +0. 69 (Latest beta) *5. 9% (Average premium of the market over Treasury) =9. 81% 3. Calculate the costs of equity using CAPM and the dividend discount model. What are the advantages and disadvantages of each method? * CAPM Cost of Equity = 5. 4% (20 year Treasury bond) +0. 69 (Latest beta) *5. 9% (Average premium of the market over Treasury) =9. 81% Advantage:| * CAPM considers only systematic risk, beta. It does not consider company specific risk. * It is useful to see an individual stock in entire portfolio. | Disadvantage:| * Some inputs are hard to reflect the situation of real world. * Relatively difficult to use co mpared to DDM| * DDM Share Price($42. 09) = Dividend($0. 48) / (re –Dividend Growth(5. 5%)) re(Cost of Equity) = 6. 64% Advantage:| * DDM only focuses on an individual stock rather than a portfolio. Always use, when calculating stock price. * Relatively easy to use compared to CAPM. | Disadvantage:| * Results are very sensitive to change when assumptions are inputted| 4. What should Kimi Ford recommend regarding an investment in Nike? RECOMMENDATION: should buy NIKE stock. NIKE stock price should be $58. 22 under the condition WACC, 9. 26%. Currently Nike stock is $42. 09. Now Nike stock is under value by $58. 22 – $42. 09 = $16. 13 per share. Works Referenced Investopedia, ND. M&A, Preferred Shares, Investopedia. [Online] Available at: http://www. investopedia. com/terms/w/wacc. asp [Accessed 1 April 2013].